Sentence examples for microarray details and from inspiring English sources
The microarray details and results are submitted to the ArrayExpress database (http://www.ebi.ac.uk/arrayexpress/), reference number E-NCMF-14.
Normalized expression values for all absolute analyses across all experimental replicates, along with other pertinent microarray details, have been deposited into the NCBI Gene Expression Omnibus GEOO) under accession number GSE14979.
These genes were identified using two approaches: 1) Based on GO term analyses of differentially expressed genes on the microarray (details in methods), 9 candidate sex determining genes were identified for further study.
The RNA was treated with DNase; 2 μg of RNA was amplified and 1 μg of aRNA was used for hybridization on the microarray; detailed information on protocols can be found at the central microarray facility website of the Netherlands Cancer Institute [ 16].
Detailed microarray methods, sample details, and data can be accessed through the Gene Expression Omnibus (GEO: GSE67899).
Platform-specific preprocessing steps were applied for Affymetrix and cDNA microarrays (detailed in online supplementary materials and methods).
Despite differences in experimental details and microarrays employed, their findings are similar to those from our more extensive analysis.
Microarray experiments were compliant with MIAME (Minimum Information About a Microarray Experiment) and both protocol details and raw data have been deposited in ArrayExpress (http://www.ebi.ac.uk/arrayexpress/) under the accession number E-MEXP-1695 E-MEXP-1695 E-MEXP-1695
Light exposure is synchronized with chemical delivery in order to synthesize high-complexity microarrays (detailed methods given in the Supporting Information).
We then immunoprecipitated Myc::FOG-1 protein and its associated RNAs using anti-Myc antibodies and probed microarrays (for details see Materials and Methods).
The top 100 clones approximately from each of the two microarray analyses detailed here, and the same for additional analyses to be detailed elsewhere, were sequenced (AGOWA, Berlin, Germany).
