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It should however be noted that in the typical microarray designs we have used in previous biological experiments we did not apply a hard ceiling at the median value for the 15-mer count but simply used the 15-mer count to guide the final oligonucleotide selection for oligonucleotides passing all the other filters [ 5].
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To validate our microarray design, we examined the distribution of mean probe set intensity and probe set variance over all assayed samples (Additional file 1) to identify problematic probe sets that failed to hybridize (extremely low mean and variance) or hybridized nonspecifically (extremely high mean and low variance).
We have developed a graphical Java-based application (Adamant) to assist with submission of Microarray designs to public repositories.
The variety of microarray applications allows for multiple, creative microarray designs and detection strategies.
Our results present ChIP microarray designs for X. tropicalis.
For example, the call rate of different resequencing microarray designs may vary considerably.
Two different microarray designs were used.
d "Validated" designs are designs that were experimentally validated, "microarray" designs are designs that had matching experimental microarray data, and "candidate" designs are simulated designs that are neither "validated" nor "microarray" designs.
Microarray designs were submitted to Agilent Technologies for production as 4 × 44K arrays.
This result illustrates the importance of experimental validation of the microarray designs.
To examine the application value of our promoter microarray design for transcription factors we studied TATA-binding protein (TBP), a key initiation factor that is known to bind to promoter regions.
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