Exact(4)
The variety of microarray applications allows for multiple, creative microarray designs and detection strategies.
Here we review different whole-genome microarray designs and applications of this technology to obtain a wide variety of genomic scale information.
Detailed information about the microarray designs and raw data files from the experiments are accessible by use of these accession numbers: GSE32869, and E-MTAB-1268 E-MTAB-1268 E-MTAB-1268
In addition, undetectable spots represent a large problem for two-color microarray designs and most arrays contain at least 40% undetectable spots even when labeled with reference samples such as Stratagene's Universal Reference RNAs™.
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Details of the microarray design and hybridization data have been deposited at the NCBI GEO repository with accession number GSE55327.
There are several important considerations regarding microarray design and analysis.
Improved versions (v2) of the X. tropicalis genome-wide microarray design and the promoter microarray design are available at the authors' web site (http://www.ncmls.nl/gertjanveenstra).nl/gertjanveenstra
Microarray design and experimentation analysis may be obtained from our recently published manuscript (Kortenhorst et al., 2008).
Microarray design and preparation, sample labeling, and microarray hybridization and scanning services were performed by the Harvard Partners Center for Genetics and Genomics (now Partners Center for Personalized Genetic Medicine).
We thank Mike Webster for help with microarray design and Luisa Dougan, Jonathan Adler, Dale Herauf, Michael Ottgen, and Jenna Gravley for synthesis and quality control of microarray chips.
All primers used in QRT-PCR are shown in Table 2. Microarray design and constructions were carried out as our previous work [28].
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