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An updated comparative genomic hybridization (CGH) microarray designed and built by Roche NimbleGen was used that includes 1,404,208 probes.
Transcriptional profile changes were analyzed from triplicate cultures by a whole genome microarray designed and synthesized by NimbleGen (Roche, Madison, WI, USA).
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LW contributed to the microarray designing and fabrication.
The oligo-microarray designed and used in the current work was developed from these previous annotated nucleotide sequences updated with lipid-responsive genes by means of a SSH approach in order to have a wide range of genes potentially regulated by nutritional deficiencies in essential fatty acids.
Rainbow trout gene expression profiling was conducted using an Agilent 4X44K high-density oligonucleotide microarray designed by Salem and coworkers.
The variety of microarray applications allows for multiple, creative microarray designs and detection strategies.
Details of the microarray design and hybridization data have been deposited at the NCBI GEO repository with accession number GSE55327.
Here we review different whole-genome microarray designs and applications of this technology to obtain a wide variety of genomic scale information.
There are several important considerations regarding microarray design and analysis.
Improved versions (v2) of the X. tropicalis genome-wide microarray design and the promoter microarray design are available at the authors' web site (http://www.ncmls.nl/gertjanveenstra).nl/gertjanveenstra
Microarray design and experimentation analysis may be obtained from our recently published manuscript (Kortenhorst et al., 2008).
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