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The microarray design for this experiment consisted of a double loop equilibrated design with dye swap.
Its schema extends the conceptual ArrayExpress design[ 44] which integrates microarray design and manufacturing, sample description, hybridization and data acquisition.
With integrated database design and accessibility, it would become possible to perform this analysis.
We thank Mike Webster for help with microarray design and Luisa Dougan, Jonathan Adler, Dale Herauf, Michael Ottgen, and Jenna Gravley for synthesis and quality control of microarray chips.
The miRNA location array was custom-made by Agilent with AMADID (Agilent Microarray Design Identifier) 014119.
ATC liaised with OGT on microarray design.
Microarray design has been described previously [39].
Microarray design and hybridization.
Gene expression profiling was performed with Agilent Whole Genome Microarrays (Agilent's SurePrint G3 Mouse GE 8 × 60K Microarray, Agilent Microarray Design ID 028005).
The pixel perfect designs can be integrated with breakpoints for smart phone and tablet viewing.
The microarray was designed with four replicates of each probe distributed across the array.
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