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The microarray design allowed us to test expression levels for 14,323 (99.1%) of the 14,460 annotated genes on the assigned chromosomal linkage groups of the Tcas_3.0 assembly [ 16].
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Our microarray design allows these variants to be rapidly tested for disease association and our results suggest that CNPs (especially those that cannot be imputed from SNP genotypes) might have contributed disproportionately to human diversity and selection.
These regions are well represented in this promoter-centric microarray design, allowing us to accurately study this portion of the genome.
The microarray design also allowed the detection of 2315 and 4060 novel transcriptionally active regions greater in length than 100 bp in unfertilized and fertilized T. castaneum eggs, respectively.
As the use of RASs reduces the distance between tiled sequences and those of extant organisms, incorporation of RASs in microarray design should allow reducing the number of sequences that need to be tiled, for a desired coverage of phylogenetic diversity.
Agilent's inkjet-like printing technology provides high flexibility for microarray design and allows convenient optimization in follow-up versions.
Improvements in microarray design now allow rapid fabrication of custom microarrays, representation of an increasingly large number of features on a single glass slide and hybridization of multiple samples on physically separated arrays on the same slide.
The primer design allowed the use of a novel DNA amplification method producing labeled, single-stranded DNA suitable for microarray hybridization.
This experimental design allows us to directly match microarray results to the a priori interpretation of gene divergence and gene absence patterns.
Carefully designed tissue microarrays create an ideal platform for biomarker discovery as their design allows direct comparisons of protein expression to be drawn between recurrence and matched non-recurrence groups.
** P value < 0.05 in qPCR, q-value < 0.05 in microarray, * P value < 0.10 in qPCR This was a novel experimental design, allowing a contrast not only between challenged and non-challenged individuals, but also varying degrees of pathology.
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