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The prognostic value of the Buck-4 signature appears similarly robust (data not shown); in contrast, the IR-7 index did not show significant outcome associations in two of the four expression microarray datasets evaluated.
Importantly, only 33.3%% (12/36) of all microarray datasets evaluated in Haibe-Kains et al. [ 6] had all the PAM50 genes available, whereas 100%% of the datasets had all three genes of the SCMGENE assay, thus highlighting another caveat of this study.
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Finally, to compare both predictors in terms of their ability to capture the main biological diversity displayed by all genes in a breast cancer microarray, we first combined 18 datasets evaluated by Haibe-Kains et al. [ 6] and identified the two main principal components (PC1 and PC2).
Actually, Mehra et al., Paulson et al., and Kim et al. interrogated published cancer microarray datasets to evaluate targeted genes, GATA3, HBP1 and CUL7, respectively [ 50- 52].
The similarity between the expression profiles of genes across the microarray dataset was evaluated via Pearson's correlation coefficient.
Annotated Stat3 and c-Myc ChIP-chip regions were compared with a published microarray dataset to evaluate developmental expression patterns of Stat3 and c-Myc bound genes.
The performance of various statistical or computational simulation methods on pair-designed microarray datasets was not sufficiently evaluated [ 27, 28].
The methodology was developed using a new Vastus lateralis (VL) muscles microarray dataset and was evaluated and validated on post-mortem brain frontal cortex (brain cortex) [ 8], kidney cortex and medulla [ 6] (Table 1).
In this study, two real-world microarray datasets were exploited two evaluate the efficiency of the proposed model.
A meta-analysis of seven microarray datasets was used to evaluate the role of the TGF-beta pathway in prostate tumorigenesis [ 48].
The relevance of the proposed methods can be evaluated using microarray datasets available in the public domain but the method is in principle applicable to other high-throughput methods.
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