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Microarray datasets (described above) were analyzed by GEOquery and Limma packages in R http://www.r-project.org/ as described previously [ 11- 13].
Control genes for the nCounter Analysis System were chosen for each cell type on the basis of consistent expression across samples in the large, cell-type-specific microarray datasets described above.
Minimum information about a microarray experiment (MIAME) criteria were met [ 56], and the complete microarray datasets described can be found at the National Center for Biotechnology Information Gene Expression Omnibus (accession number: GSE16098).
In anticipation of limited FFPE tumor section RNA availability, we prioritized the 14 microarray-derived genes comprising the Buck-14 signature into a minimal set of high priority genes showing the most robust prognostic value across the two pooled expression microarray datasets described in our previous report (training set (n = 199) and validation set (n = 75), respectively) [ 22].
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For a subset of confirmation analyses, we accessed a newer Affymetrix M430 microarray dataset (described at ).
Next, the dataset was log2 transformed, and inserted into a microarray dataset described previously [ 20], performed on RNA extracted from the exact same biopsies described above.
The microarray dataset described in this work, including.cel-files, was deposited at the Gene Expression Omnibus under the series accession GSE17475 [ 34].
Matching gene-expression and miRNA profiles of 215 sporadic breast cancer specimens were obtained by mining a microarray dataset described by Buffa et al. [ 23], in GEO (Accession number GSE22220), samples BCmicroRNA 1 to 110).
We chose three multiclass microarray datasets (detailed described in the Datasets section) for our experiments.
Using a bioinformatic approach and mining of publicly available microarray datasets, we describe an aggressive subtype of gliomas defined by a gene signature derived from REST.
Using 5 and 50 nM T4 microarray datasets, we describe the temporal transcriptional response of T4 induced metamorphosis and specifically address the following question: Does T4 concentration affect morphological metamorphosis and gene expression in the axolotl?
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