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The comparison of the gene expression profiles of our DnaJ mutants with those of other mutants deposited in the public microarray database further supported the idea that the tolerance of the DnaJ mutants to oxidative stress induced by MV is due to the trigger of a global stress response in these mutants (Additional file 6 and 7).
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The processed and loess-normalized data as well as detailed experimental information according to MIAME [ 43] were stored in the in-house microarray database for further analysis [ 44].
Next, we searched the public clinical microarray database (ONCOMINE) to further validate the 2-DE results.
The resulting jpeg and gene expression data files were deposited in a microarray database (mAdb) (http://nciarray.nci.nih.gov) and further analyzed using BRBArrayTools developed by the Biometric Research Branch, National Cancer Institute (http://linus.nci.nih.gov/BRB-ArrayTools.html).html
Datasets were further analyzed by the microarray Database system provided by the Center for Information Technology at the NIH.
Gene lists were further integrated with the tissue-dissected microarray database, FlyAtlas [ 76].
Data from GenePix Pro 4.0 was uploaded to the microarray database at the NCI/CCR Microarray Center website [ 25] for further analysis.
This analysis has provided a set of candidate marker genes, which were further investigated to define their tumour specificity using Genevestigator V3, a web-based microarray database and analysis system [ 14].
Rhodes, D. R. et al. ONCOMINE: a cancer microarray database and integrated data-mining platform.
Rhodes DR, Yu J, Shanker K, Deshpande N, Varambally R, Ghosh D et al. ONCOMINE: a cancer microarray database and integrated data-mining platform.
To investigate PD-L1 expression in NSCLCs of other cohorts, a cancer microarray database Oncomine (www.oncomine.org) was applied.
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