The microarray data were validated by qPCR.
It is based on PubMed published abstracts and aimed to close the gap between genome wide coverage of low validity from microarray data and individual highly validated data from PubMed.
To confirm the validity of microarray data gene specific mRNAs were quantified from treated and untreated cultures by quantitative real-time PCR (qRT-PCR).
We made an effort to preprocess this yeast microarray data set and examined the validity of the presented results from the perspectives of S. pombe probes sets, non-normalized expression profiles, and other considerations and supporting examples reported in our previous work.
qRT-PCR results correlated well with normalized intensity data in each organ at each time point, yielding a median overall correlation of 0.724 with the microarray data, and thereby confirming the validity of observed gene expression patterns [see Additional file 3].
These results confirm the general validity and robustness of the microarray data we present here.
Evaluation of the validity of the microarray data was performed by triplicate qRT-PCR analysis of selected transcripts (Table 1).
The real-time PCR ratios are basically similar to those of microarray data (Table 5), which confirms the validity of the array data.
To assess the validity of our microarray data, we undertook PCR analysis of 42 genes, of which 5 were used as endogenous controls to normalise the samples.
These results confirm the validity of the microarray data and indicate that our 3D reconstruction-based microdissection method was highly successful at isolating cells from targeted brain areas.
