Transcriptional signatures from purified peripheral blood mononuclear cells were derived from whole-genome gene-expression microarray data, validated by quantitative PCR and tested in independent samples.
Paralogs of miR-92a, are located in the miR-17 and miR-106a genomic clusters, which we found to be expressed predominantly in neurons, both in NT2-N and in primary human neurons (microarray data validated by qPCR; Fig. 6A, B & Table S1).
RC performed data analysis, assisted with the generation of microarray data, validated targets by qRT-PCR, wrote and revised the manuscript.
In a later study [ 164], microarray data validated by real-time PCR indicated upregulation of Spry2 in melanoma cell lines with mutations in BRAF and NRAS.
An analysis of whole genome microarray data validated that 68% of H2H genes are transcribed compared to only 44% of all human genes [ 4 ].
Thus, the 70-mer oligonucleotide microarray data validated some of the examples of AHP or BLP expression, but also indicated that some of these profiles may be false-positives.
We propose a systematic analysis of long gene expression (LGE) with a metric termed the long gene quotient (LQ) that quantifies LGE in RNA-seq or microarray data to validate neuronal identity at the single-cell and population levels.
Furthermore, it facilitates the use of microarray data to validate deep sequencing results.
Microarray data were validated by quantitative polymerase chain reaction.
The microarray data were validated by quantitative PCR (qPCR) on a subset of 10 genes.
