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In case of one-channel microarray data this approach is the same as analysis of variance except that a model is fitted for every gene.
Due to the time constraints imposed by related biological experiments that were necessary in order to confirm stress responses measured using this microarray data, this fine-tuning normalisation was not applied to the published data sets; however we include the outline of what is possible for the sake of completeness.
The HPRT1 transcript was selected as the reference gene on an empirical basis HPRT1 expression in these men (1863±111 arbitrary units, mean±SEM) and women (1881±138 arbitrary units) was similar according to the microarray data (this choice does not necessarily mean that HPRT1 expression always is constant in muscle).
As with the microarray data, this pattern appears to be unique to the Z chromosome.
With microarray data, this limit was promptly reached, for example, when a few dozen human samples were analysed.
Using publicly available DNA microarray data, this study explores folate cycle interactions at the higher level of mRNA.
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Accession codes: The sequencing and microarray data from this study have been deposited in Gene Expression Omnibus ( http://www.ncbi.nlm.nih.gov/geo/) under accession codes GSE55978 and GSE55941.
The microarray data from this study constitutes the Genomics Institute of Novartis Foundation GNFF) Human and Mouse Gene Atlases and is publicly available for exploration through the SymAtlas web‐application (http://symatlas.gnf.org).org
All microarray data in this study was described in accordance with MIAME guidelines.
The microarray data for this study have been submitted to NCBI GEO with accession GSE7376.
The microarray data of this study have been deposited in the Gene Expression Omnibus database (GSE11666).
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