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Microarray data (Tables 3, S13) and qPCR analysis (FPSC 2-fold; APSC 3-fold; Table S11) both indicate that Ahr and many of its target genes are up-regulated in FPSC and APSC, implying that the Ahr/Arnt complex is transcriptionally active in PSC.
Quantitative PCR analysis was undertaken independently in infected parental strains (days 0, 7, 14, 21, and 35 post-infection) to validate microarray data (Tables 2 and 3).
The two differentially expressed genes, pre-α/β-gliadin and γ-gliadin, were randomly chosen for validation of the microarray data (Tables 5 and 6, Figure 6).
In this paper we describe an experiment using a new database model for one of tranSMART's microarray data tables that may be more suitable for high-dimensional data storage and querying than the relational model currently implemented in tranSMART.
The microarray data tables were screened for these 22 widely used reference genes, and 20 of them could be located on the Roche NimbleGen 12 × 135 K array that was used in our experiments.
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TaqMan assay showed 5-fold more upregulation of LUM more than the microarray data (Table S4).
We selected six ovary- and ten testis-enhanced genes on the basis of our microarray data (Table S7).
Both ΔgB and ΔgH virions are capable of stimulating an NF-kB response (Table 2), a result that correlates with the preliminary microarray data (Table 1).
The real-time PCR ratios are basically similar to those of microarray data (Table 5), which confirms the validity of the array data.
These data, which confirmed the microarray data (Table 1), suggest that Src kinases activity modulate IRF8 responsive genes induced by TLR agonists.
The expression data of some of the IFN-induced genes was validated by quantitative realtime PCR and showed a good correlation with the microarray data (Table 2).
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