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Xyloglucan endotransglucosylase was up-regulated at early and mid elongation stages (3 DPA and 8 DPA), and according to the microarray data remained slightly elevated at 12 DPA.
Nevertheless, some clinically relevant microarray data remained reproducible as illustrated by a very strong correlation between the microarray expression levels of ESR1 and ERBB2 to the clinical status of ER and Her2 respectively in both FFPE and FNAB samples, which were also validated with RT-qPCR in FFPE specimens.
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All data remained confidential.
While the adoption of these standards has greatly improved the accessibility of microarray data, much of these data remain on individual authors' websites in a variety of formats; consequently, obtaining and comparing data sets remains a significant challenge.
After normalization and filtering of the microarray data, 21,671 genes remained for analysis.
The resulting microarray data contained 16,015 remaining genes.
Current efforts have shown promise in improving the analysis of short time-series microarray data, although challenges remain.
Of the 18 genes assayed, 14 confirmed statistically significant differential expression in the same direction as the microarray data, and the remaining four genes showed the same direction of expression between microarray and qRT-PCR analyses despite not being statistically different.
The data remain proprietary.
Hierarchical clustering of the remaining microarray data sets showed that experiments strongly cluster by tissue type.
Only these three rules were therefore validated on the remaining 6 microarray data sets.
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