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For example the normalization for microarray data includes background correction, normalization of signal and summarization of signal values of probe sets for a transcript.
Proper calibration of microarray data includes several tasks: Firstly it requires the determination of the model describing the basic relationship between the probe intensity and the specific transcript concentration under consideration of relevant parasitic effects which should be straightened out.
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In addition to genes involved in neurotransmitter packaging and release, the microarray data include transcripts for receptors to a remarkably wide variety of signaling molecules.
We describe in detail the methodology applied for analyzing the microarray data including differential expression as well as functional annotation of the identified symbiont genes.
Microarray data (including raw and processed data) have been deposited in National Center for Biotechnology Information's (NCBI's) Gene Expression Omnibus (NCBIGEO GSE7828).
The microarray data included those in the second model as well as those generated from experiment E4.
Using immunoprecipitation, immunohistochemistry and qPCR methods, we validate the microarray data, including altered GABAergic receptor expression in mice lacking the α9 subunit.
We assembled a large collection of microarray data, including tissue surveys, genetic mapping studies, small-molecular perturbation of cell lines, and comparisons of diseased and normal tissues, generated using several microarray platforms (Table 1; Table S1).
The microarray data included those in the initial modeling plus array data generated from experiment E3 and a published dataset monitoring the transcriptional response of the diauxic shift [22].
The microarray data included 56 complete trios from the HapMap [30] cell line panels originating from the Yoruba in Ibadan, Nigeria (YRI) and from Utah residents with ancestry from northern and western Europe (CEU).
Comparison of the two trees shows that the closely related strains based on MLST were largely grouped together by the microarray data, including strains belonging to 3 clonal complexes: ST1, ST25/ST35 and ST53/ST54.
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