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No conclusion on IL-12A transcription could be obtained from microarray data due to a high variability of the results among different donors.
The Short Time series Expression Miner (STEM) was used to cluster the microarray data due to its ability to take into account the temporal nature that is inherent in a developmental time course [33].
This type of quantitative analysis, which is very difficult to perform on microarray data due to background noise and hybridization artefacts, may facilitate a deeper understanding of differential pathways of tumor pathogenesis and the identification of highly specific diagnostic markers.
This indicates a strong technological bias of microarray data due to variation in hybridisation.
Microarray data, due to a likely over-stringent threshold, showed higher FN rate in comparison with EST data, leading to a significant underestimation of HK genes.
For microarray data, Due to the high cost of GeneChip, microarray experiments often have low sample sizes that present challenging for statistical analysis.
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However, 562 genes assessed in 5′-SAGE were not detected in the microarray data perhaps due to differential sensitivity of sequencing versus hybridization chemistry.
The RT-PCR results of the six genes studied were consistent with the microarray data, but due to the small number of genes studied with RT-PCR, data may be considered preliminary.
In fact, more recently assembled genomes like that of dog tend to have a lower quality of gene annotation translating into lower microarray data quality due to missing or incorrectly designed gene probes.
As in Cramer et al. [ 41], a note of caution should be held when examining the microarray data sets due to the likelihood of cross-hybridization of certain Vitis gene families with high similarity and are denoted in pink in Additional file 5.
OsSRP-LRS was expressed at a high level of mRNA at several developmental stages and in a range of tissues (consistent with the Rice GE: Gene Expression Analysis microarray data), possibly due to an as yet undefined constitutive function of the gene product.
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