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For convenience, we use the term "gene" instead of "gene feature" in the microarray data descriptions.
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For detailed DNA microarray data description and analyses see [ 11].
In the database design, we have taken into consideration the latest MIAME recommendations from the MGED Society regarding microarray data description [ 39].
Raw microarray data and the description of the array have been deposited in the Gene Expression Omnibus Database, under the series number GSE32716.
Miami compliant microarray data -including a detailed description of the experimental design and each hybridization experiment- were deposited in the Gene Expression Omnibus (GEO) database with the superSeries unique identifier GSE23052.
For all of the statistical analysis beyond the initial description of datasets, microarray data were normalized (Dataset S1) and a subset of the 12 microarray data (10 from melanoma cell lines and 2 from normal human melanocytes) was obtained by filtering to require each gene probe to have at least one observation in the expression intensity resulting in a 'present' call from all 12 samples.
The microarray data presented here provides the first description of changes in the B. pseudomallei-infected host cell metabolism particularly the glycolytic and TCA pathways.
Here, an explicit description of the microarray data, the number of and types of samples in the database, and how "absolute transcript abundances" are calculated is necessary to understand the rest of the paper.
Significance codes are defined by the intervals: '***' < 0.001 ≤ '**' < 0.01 ≤ '*' < 0.05 Microarray data for RT-PCR validated microRNAs, see description of Table 1.
Please see the Partek website http://www.partek.com/ for a more detailed description of statistical analysis of microarray data using the Partek Genomic Suite applications.
A detailed description of the honey bee microarray data set is presented by Whitfield et al. and Rodriguez-Zas et al. [ 17, 18].
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