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Largely, expression profile of NCX genes obtained from microarray data corroborate with MPSS data.
Our microarray data corroborate those of Tran et al. [ 27], suggesting the involvement of a different NAC domain-containing transcription factor (at5g08790) in expression of FeSOD, as well as several other genes known to exhibit NAC-dependent expression.
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In view of the apparent benefit of using other microarray data to corroborate and refine gene lists, we also evaluated the differential expression patterns of the 52 putative photorespiration genes across the 40 microarray data sets described above (Figure 6C; Table S11).
Thus, in the case of MUC5AC, the microarray data were corroborated by protein expression analyses.
Selected microarray data were corroborated by other procedures such as reverse transcriptase PCR analysis of mRNA, and flow cytometry.
The microarray data were successfully corroborated for most time-points with the exception of the SANT/MYB transcription factor gene (probeset ID Ghi.1711.1.S1_s_at) at 8 DPA.
Digital northern data based on ESTs have corroborated microarray data in other recent studies [29].
Our analysis of the Stransky bladder carcinoma microarray data [ 25] corroborated these findings inasmuch as HBEGF transcript levels increase in infiltrating tumors with respect to superficially growing lesions.
Together, these quantification strategies for Q-RT-PCR data procedureg procedure, which focused on minimal variation, ought to significantly facilitate internal control evaluation and selection for Q-RT-PCR when corroborating microarray data.
Q-RT-PCR has also become one of the gold standards for both pathogen detection and gene expression studies and is the method of choice for corroborating microarray data [ 4].
To corroborate the microarray data gene-specific qPCRs were performed.
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