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The heat shock gene cluster was obtained by clustering stress-response microarray data conducted by Kilian et al. [41].
JA processed microarray data, conducted statistical data analysis, and developed new algorithm and software.
PLK conducted the RWPE1 experiment, performed statistical, functional and cluster analysis of microarray data, conducted validation of target genes using QRT-PCR and prepared the manuscript.
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v) Meta-analysis of the differentially expressed genes Affymetrix® wheat microarray expression data conducted in a wide variety of growth stages and tissues were available online.
ES participated in necropsy, processed tissues, performed microarray experiments, analyzed data, conducted qPCR validation and drafted the manuscript.
EB isolated RNA and developed the G. holbrooki cDNA library using the MINT cDNA synthesis and TRIMMER kits, isolated RNA from the tissue samples used for the microarray analysis, conducted the cRNA amplification and microarray hybridization, analyzed the microarray data, and conducted the qPCR analysis.
To independently assess the microarray data, we conducted QPCR on RNA from purified spermatocytes and spermatids.
First, a meta-analysis of two published microarray data was conducted to obtain a list of 343 differentially expressed genes specific to lung AC.
The presence or absence of a gene was coded as binary data with gene presence as 1 and absence as 0. Hierarchical clustering on microarray data was conducted using Cluster 3.0, and trees were represented using TreeView [28].
Normalization of the microarray data was conducted using RMA.
All other experiments, including analysis of the microarray data, were conducted by HDR.
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