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MFM collected the samples, performed the analysis of the microarray data, carried out the real-time RT-PCR experiments, and drafted the manuscript.
HK collected primary mammary cell samples, isolated RNA, carried out qPCR analyses, helped analyse microarray data, carried out fat pad transplants and analysed transplant results.
JHL participated in the design of the study, analyzed microarray data, carried out western blot experiments with human tissue lysates, and drafted the manuscript.
IZ participated in the design of some experiments and the validation of Microarray data, carried out phenotypical and biochemical analyses and contributed to the manuscript writing.
CA contributed to experimental concept and design, cultured and treated cells, purified RNA, performed analysis and interpretation of microarray data, carried out network analysis, drafted and revised manuscript, gave final approval for version to be published.
The tissue-specific selection of reference genes using microarray data carried out in similar conditions allows to identify novel genes having higher expression stability and a more suitable expression range than commonly used reference genes.
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YYM was responsible for performing database searches, analyzing microarray data, carrying out cell proliferation assays, and preparing the first manuscript draft.
Quality control and filtering of microarray data was carried out with GeneSpring GX software (Agilent Technologies).
For further secondary validation of the microarray data we carried out western blot analysis for several proteins.
Analysis of microarray data was carried out by the WebGestalt Program, maintained by Vanderbilt University and available online at http://bioinfo.vanderbilt.edu/webgestalt/.edu/webgestalt/
To validate microarray data, we carried out real-time RT-PCR focusing on nine genes strongly associated with the DSS-gene signature, using 15 patients samples (five from each disease phenotype subtype: DF, DHF and DSS).
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