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Here we describe the experimental methods and analysis of the microarray data available in Gene Expression Omnibus (GEO) under GSE45165.
From the rice Affymetrix microarray data available in the NCBI gene expression omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/), we used 983 arrays consisting of 595 indica and 388 japonica samples.
We therefore compiled a large collection of microarray data available for a wide range of experimental conditions for S. cerevisiae from the M3D database [26] (experimental conditions are indicated in the Table S3).
While annotation is useful and in some cases necessary for some supervised analysis, the practice of insisting on detailed metadata in the process of making raw microarray data available may actually limit the amount of raw data deposited.
In contrast to the RT PCR data, we found no significant variations in the expression levels of the RASGRF2 mRNA in the microarray data available at the Oncomine site, indicating that this gene is not commonly deregulated at least in the case of the hematopoietic tumors selected in this study.
Microarray data, available at ArrayExpress at www.ebi.ec.uk/arrayexpress/ under accession number E-TABM-163, were normalized as described [5] and filtered based on detection call (by removing the probe sets called "absent" throughout the experiment), signal intensity (by removing genes with low-maximum expression level <50) and amplitude (by eliminating peak-to-trough variation <1.65).
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RNA isolated from HS and untreated cells was reverse transcribed, labeled and hybridized to NimbleGen expression microarrays (data available in GEO under GSE19745).
The microarray data was available to all authors of the article and is available upon request.
Microarray data are available via GEO (https://www.ncbi.nlm.nih.gov/geo/) under accession number GSE107521.
Primary microarray data is available in the National Center for Biotechnology Information's Gene Expression Omnibus public database.
All microarray data are available from the Gene Expression Omnibus database under accession code GSE50438.
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