As expected, the microarray data revealed approximately fourfold upregulation of transcripts encoding Brg1, and this result was independently confirmed by quantitative real-time polymerase chain reaction (qRT-PCR).
These calculations indicated that the 70-mer oligonucleotide microarray data generated approximately twice as much signal variation among replicates than the Affymetrix platform [see Additional file 6].
Expression data for three to six animals per time point per treatment group dosed for 1, 3, and 5 days were analyzed and compared with a contextual reference database of microarray data for approximately 600 compounds.
For this reason, the present study focuses on murine microarray data containing approximately 400 samples, all obtained using the Affymetrix MG-U74Av2 platform (Table 1) [ 3, 6, 25- 39].
Ross et al (2000) and Scherf et al (2000) have reported microarray data on approximately 8000 genes in the cell lines of the NCI panel, including β 2-m.
The analysis of the microarray data revealed that approximately 4.5% of the A. gambiae ESTs were up or down-regulated by at least 1.5-fold.
One of the observations from our microarray data was the approximately 20-fold upregulation of SERPINB5 and almost 60% downregulation of BIRC5 genes.
In silico expression analysis based on microarray data found that approximately 5% of these genes are transcriptionally more active in somatic embryos than in actively dividing callus and non-dividing leaf tissues.
We next binned the transcripts into groups of highly, moderately and lowly expressed transcripts (based on the microarray data), with an approximately equal number of transcripts in each bin.
Furthermore, a wide survey of available Arabidopsis microarray data suggested that only approximately 40 transcripts encoding putative cation transporters showed a significant response to salt or drought stress, with less than a 10% overlap between studies [ 58].
In this work we used cDNA microarray data containing 37,632 elements that represents approximately 11,000 unique genes and approximately 12,000 genes without identification [ 1, 17].
