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Our development stage specific microarray data also complements the expression profile of CAX genes as reported previously (Kamiya et al. 2005), which shows high transcript levels of OsCAX1a (OsCAX2), OsCand (OsCAX3) and OsCAX3 (OsNCX9) while OsCAX1b (OsNCX10) and OsCAX1c (Oshow5) show very low transcript level in different plant tissues.
Similar to these results, our microarray data also provided some novel insight to identify pathogenicity factors.
The microarray data also showed the Ehd3 gene to be strongly up regulated in GEC.
The microarray data also revealed increased transcription of the tagA, tagG and tagB genes concerned with teichoic acid biosynthesis.
The microarray data also shows that OHHL modulated the expression of genes involved in metal ion transport and storage.
While not in this gene cluster, our microarray data also identified Rv3849 (espR), a recently characterized secreted regulator of ESX-1 [49], as under-expressed in H37Ra.
Our microarray data also correlate with recently published data on the role of UCH L1 in B-cell proliferation and invasion [21].
Furthermore, deletion of a down-regulated transcription factor, from the ΔpdeH microarray data, also showed reduced pathogenicity, similar to that of the ΔpdeH mutant.
Differential expression of relatively fewer miRs when compared to the large number of differentially regulated genes in microarray data also suggests the possibility of targeting more than one gene by each miRNA.
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K562 and sUHR microarray data were also analyzed using Microarray Suite 4.0 in order to calculate PM-MM values for each transcript probe set.
We performed a quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis to confirm the microarray results using total RNA extracted from floral bud clusters up to stage 7. Similar to previous microarray data, we also detected a reduction in YUC4 transcript levels in the ag-1 mutant compared with the wild type (Fig. 2a).
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