Suggestions(2)
Exact(1)
3. The cell type enrichment analysis of age-regulated genes was performed independently for mice and humans, and included all genes that met the above microarray criteria and were also present in the list of cell type-enriched genes in the Allen Brain Atlas (Table S8).
Similar(59)
The overlap between the two approaches was 70% under the microarray criterion of log2 ratio > = 1 (Fig. 3C, 316 RTCs, P = 6.1 × 10-5) and 80% using the criterion of 1.5 (Fig. 3D, 148 RTCs, P = 10-4).
Two large microarray investigations met these criteria and were selected for examination of the presence of neuropeptides and for evidence of differential expression between conditions.
Subramanian and colleagues selected 1875 of almost 28 000 genes or ESTs (expressed sequence tags) clusters represented on cDNA microarray that passed filtering criteria and used it for further analysis.
In two independent groups of adults with epilepsy and co-morbidities, we identified copy number variants judged to be of pathogenic significance in 13.5% (7/52) and 20% (5/25) respectively, noting that different criteria and microarray platforms were used, giving an overall yield of 15.6%, in keeping with the literature [1].
We call upon the microarray community to consider these criteria and establish a standard protocol for etablishing molecular equivalence.
Using sequence similarity criteria, and data from microarrays studies identifying genes of unknown function that are expressed late in sporulation [ 49, 50], we compiled an expanded list of 73 genes (see Additional files 1 and 2), that includes genes we regard as strong candidates for coat protein genes [ 48, 49].
Spearman rank correlation analysis was first performed for a total of 5,560 common transcripts that passed the RNA-seq and microarray filtering criteria (detailed in Methods).
Probes showing the best combination of thermodynamic criteria and strain coverage were selected for microarray manufacturing (Fig. 1f).
Using a commercial expression microarray platform and standard filtering criteria, 4,332 unique genes were found to be variably expressed within this set of histologically similar, early-stage ER-positive breast cancers.
The MIAME (Minimum information about a microarray experiment) criteria 5 formulate the necessary conditions for verifying and reproducing results of microarray data analyses.
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