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In the chip operation, after the continuous flow PCR was achieved in the first microfluidic chip, the product was directly introduced into a hybridization chip integrated with microarray containing the immobilized DNA probes.
Tag sequences from each T and N matched population were amplified, labeled with different fluorochromes, mixed and hybridized to a DNA microarray containing the complete repertoire of tag sequences.
Tissue microarray containing the tissue cores was then cut into 4- μm sections for hematoxylin and IHC staining.
Subsequent CGH analysis on a cDNA microarray containing the subtracted clones allows detection of differentially expressed genes which are amplified at the DNA level.
For example, Narusaka et al. studied the crosstalk between abiotic and biotic stress responses using a cDNA microarray containing the genes in the cytochrome P450 superfamily [ 9].
In contrast, our study utilized a microarray containing the genome-wide set of human gene promoters to search for potential binding sites of HBc, and identified over 3000 putative functional targets.
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The diagnostic DNA microarray contains the species-specific probes of 15mer oligonucleotides designed based on the sequences of 23S ribosomal DNA.
Each microarray contains the reverse complement of 866 miRNAs and mature star sequences as published in the Sanger mirBase 12.0.
The sample series for each microarray contains the raw data (median signal) of each Alexa637 and Alexa555 channel as well as the normalized data (log2 (Alexa637/Alexa555 ratio)).
miRNA from the human embryonic tissue was isolated using the miRNeasy Kit (Qiagen) and sent to Exiqon for a miRCURY LNA Universal RT microRNA PCR; the microarray contained the 59 strongest-expressed miRNA from the RNA sequencing.
This microarray contains the Agilent 44 K (ID 014850) probe set incorporating human gene expression protein-coding probes as well as non-coding probes; with the probes targeting exonic regions, 3′UTRs, 5′UTRs, as well as intronic and intergenic regions [ 8].
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