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The authors would like to thank David Barber and Bill Farmerie for their guidance with the microarray construction, with additional thanks to David Barber for his help with the design of the exposure study.
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The insert ends of clones that fulfilled the cutoff values mentioned were recovered from the genomic library used for microarray construction, sequenced with the M13-pUC18 primers and assembled as described, a strategy that is not affected by insertions, deletions and substitutions between the MCAN/ES/98/10445 and the genome-sequenced MCAN/ES/98/LLM-877 isolates [ 26].
All 117 patients with EAC used for the tissue microarray construction were treated with esophagectomy at Strong Memorial Hospital/ Universityof Rochester from 1997 to 2005.
All the 110 patients with esophageal adenocarcinoma used for the tissue microarray construction were treated with esophagectomy at University of Rochester Medical Center/Strong Memorial Hospital from 1997 to 2005 without pre-operation neoadjuvant therapy.
Reference DNA (0.5 μg from the mix used for microarray construction) was labeled with Cy3-dUTP.
FG helped with microarray construction and liquid culture experiments.
A detailed description of the probe cDNA library generation and characterisation, along with the microarray construction, is provided elsewhere [ 2].
Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study.
Tissue microarray construction was completed in the Liver Cancer Institute of Zhongshan Hospital with clinical HCC specimens obtained from a cohort of 48 patients.
Oligonucleotide arrays were constructed photolithographically by an oligo DNA microarray construction service (NimbleGen).
Tissue microarray construction and high-throughput analysis of tissue samples were performed in accordance with Institutional Guidelines.
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