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In the cases of the hamartochrondroma control group, cores with diameters of 0.60 cm and 0.24 cm were obtained from paraffin blocks using a prototypical self-made manual tissue puncher (a device for tissue microarray construction developed by M.W).
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Oligonucleotide arrays were constructed photolithographically by an oligo DNA microarray construction service (NimbleGen).
Formalin-fixed, paraffin-embedded tumor specimens were used for tissue microarray construction.
Cell lysates were stored at –80°C prior to microarray construction.
A total of 7209 ESTs from three intestinal cDNA libraries were chosen for the microarray construction.
Details about microarray construction, clones list, probes preparations, hybridizations and washes have been previously described [65].
Performed microarray construction and analysis.
AT participated to the microarray construction.
Oligo microarray construction was performed by Agilent Technologies Inc.
PB contributed to DNA microarray construction.
FG helped with microarray construction and liquid culture experiments.
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