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Each BAC clone was verified by fluorescence in situ hybridization (FISH) for its chromosomal location prior to microarray construction and validated for use in FISH to visualize chromosome abnormalities identified by the microarray.
These studies push the lower limit of probe size in genome-scale unmodified oligonucleotide microarray construction and demonstrate a versatile, cost-effective and reliable method for molecular barcode analysis.
Performed microarray construction and analysis.
Microarray construction and hybridization were previously described [ 23].
FG helped with microarray construction and liquid culture experiments.
Tissue microarray construction and immunohistochemistry protocol were described previously [ 13].
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Prostate cancer patients were selected for the study according to the following criteria: availability of both tumour and normal tissue for each patient, presence of adequate amount of stroma in both normal and tumour tissues for efficient selection for tissue microarray construction (TMA), and absence of chemotherapy or radiotherapy.
For microarray construction, qPCR and RACE (rapid amplification of cDNA ends) samples were collected from 36 potted three-year-old cuttings placed in an outdoor location at the FTBC.
TS participated in the microarray construction, study design and manuscript writing and cultured the flies and extracted the RNA for the microarray study.
Sampling for sequencing and tissue microarray construction was limited, and intratumoral heterogeneity was not addressed.
AK performed the moult staging, animal husbandry and sampling, library preparation, sequencing, bioinformatic analysis of the ESTs, microarray construction, microarray hybridizations, and interpretation of the data, contributed to the experimental design and drafted the manuscript.
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microarray interpretation and
microarray analysis and
microarray hybridization and
microarray hybridisation and
microarray expression and
microarray assay and
microarray dataset and
microarray printing and
microarray quality and
microarray format and
microarray development and
microarray preparation and
microarray platform and
microarray slide and
microarray technology and
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