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All cDNA sequences represented in the sea bass microarray consist of public mRNA sequences or ESTs produced through 5' end sequencing of clones from directional cDNA libraries.
The microarray consist of 60-mer DNA probes synthesized in situ, which represent 723 human and 76 human viral miRNAs from the Sanger database v 10.1.
Negative controls added to this microarray consist of oligonucleotides derived from the gene sequences for the green fluorescent protein of Aequorea victoria, the lactose permease of Citrobacter freundii, and the chlorophyll synthase from Arabidopsis thaliana.
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We then analyzed gene expression profiles in the treated cells using a cDNA microarray consisting of 18,432 genes.
A DNA microarray consisting of 221 genes with 390 oligonucleotide probes was designed to identify characteristic genes or gene alleles of S. aureus.
A total of 17,329 S. mansoni DNA sequences were used to design a microarray consisting of 7335 parasite elements or approximately 50% of this parasite's transcriptome.
The impact of substrates for probe immobilization was studied using a microbial diagnostic microarray consisting of probes designed against the pmoA genes of methanotrophs and functionally related bacteria.
A microarray consisting of oligonucleotide probes targeting variable regions of the 16S rRNA gene was designed and tested for the investigation of microbial communities in compost.
A 4 × 44 K 60-mer oligo microarray consisting of 43,833 probes covering 42,440 unigene sequences and 1417 positive and negative Agilent control sequences was designed and used to analyze the expression profiles of four tea plant clones.
The microarray consisted of a 4 × 4 matrix, including the five pathogen-related antigens and human IgG positive control in duplicate (Fig. 2).
However, the expression and significance of nucleolin in pancreatic ductal adenocarcinoma (PDA) have not been studied.We used a tissue microarray consisting of 1.0-mm cores of tumor and paired nonneoplastic pancreatic tissue from 69 pancreaticoduodenectomy specimens with stage II PDA.
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