Sentence examples for microarray confirmation by from inspiring English sources

The exception to microarray confirmation by real-time RT-PCR was MCF2L, a gene whose variability in expression was notably high.

The general data from microarrays require confirmation by more precise investigation to confirm and specify the activity of flax-derived cannabinoid.

The review discusses a possible molecular basis for predictive tissue biomarkers such as melanoma thickness, ulceration and mitotic activity, and provides a list of promising new biomarkers identified from tissue microarrays that needs confirmation by independent, prospectively collected clinical data sets.

A set of 11 genes present on the microarray was chosen for confirmation by quantitative RT-PCR, 8 of which were significantly differentially expressed between the 16 h fasting state and the Fed state.

Using oligonucleotide microarrays, another confirmation derived by a study in which the expression profiles of primary prostate cancer, adjacent normal tissue and normal tissue from tumour free donors were compared [44].

To validate these results, several genes showing strong up- or down-regulated expression levels based upon the microarray results were selected for confirmation by real-time quantitative PCR analysis.

In the current study, one gene without known annotation but identified as very highly differentially expressed by microarray analysis was chosen for further confirmation by qPCR along with others that did not easily fit into a functional category.

Figure 3 shows the confirmation by RT-qPCR of the microarray data.

Briefly, interactions were assessed using a peptide microarray followed by confirmation of positives and measurement of binding affinities by fluorescence polarization (FP), a high-quality affinity measurement method (Stiffler et al., 2007).

However, we favour using these microarray data as primary screens of gene expression followed by subsequent confirmation by gene-specific RT-PCR studies.

Our results also raise the possibility of using microarray CGH results to identify putative small CNVs and SNP for confirmation by high-throughput sequencing or other methods.