In this study, using newly generated isoform-specific antibodies, immunohistochemistry (IHC) was performed on a tumor microarray comprised of 144 specimens.
We report the development of a microarray comprised of proteins expressed from 96% (1433/1493) of the predicted ORFs encoded by the genome of the zoonotic pathogen Bartonella henselae.
We used our RAET1G1 specific antiserum to probe a tissue microarray, comprised of 344 cores from 172 donors, representing 35 normal tissues (Table 1).
A protein microarray comprised of 4032 spots from 1433 ORFs spotted in duplicate, with positive and negative controls was fabricated, as described in Methods.
From data obtained with a microarray comprised of the vaccinia virus proteome, we previously reported [16] that human vaccination resulted in the induction of IgG that was specific for eight vaccinia proteins: H5R, C3L, I3L, A27L, D13L, I1L, H3L, and A33R.
Utilizing antigens identified through tissue analysis with IMS, we also examined serum antibody responses by a focused proteome microarray comprised of Burkholderia proteins.
To accomplish this, a DNA microarray comprised of over 41000 oligonucleotides was designed and constructed using in situ inkjet oligonucleotide synthesis.
A lectin microarray comprised of 38 lectins as described in our previous study was used to profile the glycan structures of the proteins biotinylated via Staudinger ligation [ 35].
By using this EST resource, we have also developed a low redundancy cDNA microarray comprised of 9,690 sequences, which, in combination with multiple sequence annotations, will be a powerful tool to investigate transcriptome modulation in spruces and conifers.
A microarray comprised of approximately 300 recombinant proteins from B. mallei, including GroEL as well as control proteins from the gram-negative pathogen Yersinia pestis, was used to measure serological immune responses to infection.
