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In this study, using newly generated isoform-specific antibodies, immunohistochemistry (IHC) was performed on a tumor microarray comprised of 144 specimens.
We used our RAET1G1 specific antiserum to probe a tissue microarray, comprised of 344 cores from 172 donors, representing 35 normal tissues (Table 1).
A protein microarray comprised of 4032 spots from 1433 ORFs spotted in duplicate, with positive and negative controls was fabricated, as described in Methods.
We report the development of a microarray comprised of proteins expressed from 96% (1433/1493) of the predicted ORFs encoded by the genome of the zoonotic pathogen Bartonella henselae.
From data obtained with a microarray comprised of the vaccinia virus proteome, we previously reported [16] that human vaccination resulted in the induction of IgG that was specific for eight vaccinia proteins: H5R, C3L, I3L, A27L, D13L, I1L, H3L, and A33R.
Utilizing antigens identified through tissue analysis with IMS, we also examined serum antibody responses by a focused proteome microarray comprised of Burkholderia proteins.
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With a procedure designed to minimize false positive identifications, analysis of gene expression microarrays (comprised of ≈25,000 genes) identified a small number (<20) of genes that were differentially expressed across animals that exhibited fast and slow aggregate learning abilities.
Archival formalin-fixed and paraffin-embedded primary tumor tissue was used to generate tissue microarrays comprised of two 2 mm cores from each tumor, as previously described [ 21].
Breast tissue microarrays comprised of normal, ductal carcinoma in situ (DCIS), invasive and metastatic samples were purchased from US Biomax, Inc. (Rockville, MD, USA).
In the validation study, protein-protein interaction assays were performed using pools of proteins probed against yeast protein microarrays comprised of more than 4000 yeast proteins, followed by successful deconvolution of expected individual interaction pairs.
Similarly, Wang et al.[ 90] used glycan microarrays comprised of Globo H, a cancer-associated carbohydrate antigen highly expressed on breast cancer cells and other related structures, for quantitative analysis of their respective autoantibodies present in the plasma of breast cancer patients and normal blood donors.
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