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Microarray chips were scanned using a DNA Microarray Scanner (Agilent Technologies).
The microarray chips were scanned with GeneChip® scanner (Affymetrix).
cDNA and microarray chips were prepared and hybridized by the Bioinformatics Unit of Tel Aviv University.
Affymetrix mouse expression array A430 microarray chips were used for gene expression detection.
Affymetrix Mouse 430 2.0 microarray chips were used to monitor the expression level of 45,101 probe sets representing over 39,000 transcripts and variants from over 34,000 mouse genes (Affymetrix).
Microarray chips were analyzed by the GCOS (GeneChip Operating Software) from Affymetrix with the default settings except that the target signal was set to 500 and used to generate a microarray quality control and data report.
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Currently, several platforms of microRNA microarray chips are commercially available.
Quality control of microarray chips was performed using the AffyQCReport software [77].
The R2 for the replicate microarray chips was found to be R2>0.98, which indicated negligible total variability between replicates.
DNA microarray chips are constructed on a solid surface, which provides reactive groups to which can be attached nucleic acids, called probes [8].
Quality control of microarray chips was performed using the AffyQCReport software (Gautier et al, 2004).
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