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Individual Infinium microarray chips used were input into ComBat as batches.
The microarray chips used contained probe regions that detected 1135 (Toray Industries) miRNA transcripts listed in Sanger miRBase Release 17.0.
Only genes that were overlapping between different types of microarray chips used in our study were included in the analysis.
We realized that the Affymetrix oligomer microarray chips used in this study contain genes from O. sativa, but not from O. longistaminata.
Furthermore, the greatly increased number of microarray chips used here (increased from 14 to 48) allowed for the use of a more robust ANOVA-based statistical analysis.
The microarray chips used for this study were the commercially available Affymetrix GeneChip® Cotton Genome Microarray (Affymetrix Inc., Santa Clara, CA), comprising 239,777 probe sets representing 21,854 cotton transcripts from a variety of EST databases.
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Initial analysis of the DNA microarray chips using the Affymetrix software indicated that approximately 15,000 of the 54,675 sequences recognized in the array (i.e. 27.5%) are expressed by resting human neutrophils.
In addition to RMA, we also employed the other two different methods, MAS5 and GC-RMA, for the probe normalization to analyze 66 Affymetrix microarray chips using lung adenocarcinoma RNA samples.
The differentially expressed genes were used to compare against a reference set of 21,218 genes on the microarray chip used in Papapetropoulos et al. (hgu133plus2).
We further filtered by the presence of the predicted target gene on the microarray chip used in the experiment, information available at GEO datasets.
The AppChip2 microarray chip used in this study is an evolved version of the AppChip1 chip, and like its predecessor, was a part of the A. pleuropneumoniae 5b L20 genome sequencing project [101].
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