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Different types of microarray chips have been designed for different investigations, being expression chips the most common application.
Although microarray chips have been used for distantly related taxa with varying success [ 19] the usefulness of a de novo reference transcriptome for studies of related taxa has not yet been determined.
Since all of the probes on the microarray chips have been well characterized, the hundreds and thousands of genes in the subtracted amplicons can be determined by a single hybridization.
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The advent of high-throughput technologies, such as transcript microarray chips, has allowed simultaneous interrogation of multiple molecular components at any given time.
The quality and consistency of microarray chips has improved since the HESI experiments were conducted, agrees Brenda Weis, who along with William Suk administers the Toxicogenomics Research Consortium (TRC), a component of the NCT.
The development of the DNA microarray chip has been driven by modern techniques of microelectronic fabrication, miniaturization and integration to produce what is referred to as "laboratory-on-chip" devices.
Our cell microarray chip has been improved to allow the regular dispersion of an erythrocyte suspension in a nuclear staining fluorescence dye in the microchambers, with the formation of a monolayer, and analysis with a microarray scanner for detection of the presence of fluorescence-positive nuclei in erythrocytes (Fig. 1a-c).
The microarray chip had probe sets for 12,625 features, which were normalized and summarized using the Robust Multi-array Average (RMA) method [ 42] on perfect match probes only.
Gene expression microarrays (gene chips) have revolutionized biology by generating vast amounts of data roughly quantifying the level of mRNA expression for thousands of genes in a single sample.
A number of techniques, ranging from differential display and nucleic acid subtraction to serial analysis of gene expression, expression microarrays and gene chips, have been used to the discovery of such aberrantly expressed cancer-related genes [ 1].
DNA microarrays, also called DNA chips, have stoked excitement in the research community because they can test the activity of many genes at once.
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