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Commercially available microarray chips for mouse genes (Agilent MouseGE 4 × 44 K, Agilent Technologies) were used.
Finally, 1.25 μL of each diluted sample was added to 5 μL PCR reaction mixture and then uploaded onto microarray chips for dPCR analysis.
Earlier studies have successfully used this version of microarray chips for elucidating genes involved in zebrafish embryogenesis [52], liver tumor progression [81] and Hedgehog signaling [53].
The microarray chips for this study were constructed by Agilent Technologies.
Here, we describe a novel method to amplify oligos from microarray chips for direct use in MAGE to perturb thousands of genomic sites simultaneously.
This discrepancy may reflect higher sensitivity with real-time qPCR or suboptimal probe design in microarray chips for the assessed cell culture systems.
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Cultured Plasmodium falciparum strain 3D7 was used to examine the potential of the cell microarray chip for malaria diagnosis.
Using a cell microarray chip for evaluation of the efficacy of antimalarial drugs, more sensitive and high throughput evaluation is anticipated.
In our previous study, we developed a single-cell microarray chip for the analysis of antigen-specific single B-cells [14].
The potential of the cell microarray chip for the detection of malaria-infected erythrocytes was shown, it offering 10 100 times higher sensitivity than that of conventional light microscopy and easy operation in 15 min with purified erythrocytes.
In conclusion, we developed a high density protein microarray chip for antibody specificity evaluation.
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