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Currently, several platforms of microRNA microarray chips are commercially available.
DNA microarray chips are constructed on a solid surface, which provides reactive groups to which can be attached nucleic acids, called probes [8].
Pairs of orthologs probe sets from different Affymetrix microarray chips are stored.
However, no commercial microarray chips are available for sorghum to analyze the expression profiles.
Thus, currently available commercial microarray chips are not enough to cover all rice genes and to outline their expression profiling.
Basically, DNA microarray experimental process represents the process by which DNA probes attached to the surface of microarray chips are used to hybridize with a labeled cDNA sample, in order to quantify functional gene products.
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The microarray chips were scanned with GeneChip® scanner (Affymetrix).
Quality control of microarray chips was performed using the AffyQCReport software [77].
cDNA and microarray chips were prepared and hybridized by the Bioinformatics Unit of Tel Aviv University.
Affymetrix mouse expression array A430 microarray chips were used for gene expression detection.
The R2 for the replicate microarray chips was found to be R2>0.98, which indicated negligible total variability between replicates.
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