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Microarray chips and reagents used in the experiment of a given colony were from the same Affymetrix batch.
Thirdly, for dPCR analysis, RMs were diluted to about 18 copies/μL (by about 109 times dilution), then automatically uploaded onto the microarray chips and quantified.
This will allow for analysis of multiple parallel samples, essentially lowering the cost of microarray chips and sequencing reactions.
RLJ provided microarray chips and contributed to the discussion.
Scanning of the microarray chips and acquisition of data from scanned images were as previously described [ 22].
In the present study, 36,000 oligonucleotide probe sets were included in microarray chips, and 550 selected common significant genes were mapped.
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Although the cost of a cell microarray chip and the dye is less than US$1.0,, the cost is US$400 for the isolation of leukocytes with LeukoLOCK™.
Recently, Jin et al. improved this single-cell microarray chip and developed a new system that can directly detect the secretion of antibodies by single cells [15].
Malaria-infected erythrocytes in SYTO 59 for staining of malaria parasite nuclei were dispersed on a cell microarray chip and then scanning images were obtained (Fig. 4a-h).
Prior to microarray chipping and real time quantitative RT-PCR analysis, the isolated RNA samples were treated with DNA-free™ recombinant DNase I (Ambion Inc, Austin, TX) according to the manufacturer's instructions to remove any potential genomic DNA contamination.
Each of these sample pools was used to probe a separate microarray chip, and thus the mean expression values for each of the three genotypic groups analyzed is the average of 70 individual larvae.
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