Each microarray chip includes two in-slide replicates (spot replicates).
This means that a custom-made microarray chip that includes these consensus genes should be designed, and quality controlled samples from several laboratories included.
The antibodies used for protein microarray chip analysis include rabbit polyclonal anti-ERCC1 (FL297) (Santa Cruz), anti-ERCC1 (8F1) (Abcam), and Anti-ERCC1 (4F9 and 2E12) (OriGene Technologies Inc).
The porcine chip referred to as SLA-RI/NRSP8-13K chincludesdes a newly designed SLA-RI oligonucleotide set, the Qiagen-NRSP8 microarray oligonucleotide set [ 17] and a series of positive and negative control elements (Table 1).
This experiment was performed on the 8 K Arabidopsis microarray chip that does not include all genes used in our analysis; it does however include PSY and a number of other genes under investigation and thus provides a reasonable representation of the biosynthesis pathways being examined [ 64, 65].
Each miRNA microarray chip contained 1,199 probes, including 703 identified human miRNAs and 393 predicted miRNAs.
Extra blood chips included.
The microarray chip used in our study included three different probe sets for TRAIL which further increased the specificity and reliability of the data.
More specifically, a combination of ATF3, CLDN1, CXCL1, CYR61, EGR1, FN1, IL-8, MUC1, PLAU, THBS1 and TNC determine the LPA-positive clusters (Figure 1, Figure S2, Figure 2A and 2D, and data not shown) when these genes are included on the microarray chip.
