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1.5 μg cRNA was then hybridized to each microarray chip following manufacturer instructions.
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Microarrays consisting of 28585 gene probes identified gene expression changes equating to ~4% and 18% of genes on the chip following drought and heat stresses respectively.
One microgram of each labeled DNA was hybridized onto one segment each of three replicate custom Agilent 8x15K Comparative Genomic Hybridization (CGH) microarray chips (Agilent Technologies, Santa Clara, CA), following the manufacturer's instructions.
The glutathione sensor chips were prepared from CM5 chips following standard amine-coupling protocol.
Results from immunostaining and chromatin immunoprecipitation (ChIP) coupled with DNA microarray (chip) (ChIP-chip) analysis in Arabidopsis suggest that methylation of H3K9 and H3K27 is important for chromatin structure and gene regulation.
For the genes that are represented by more than one probe on the microarray chip, we selected those that have at least one probe following the above criteria.
Total RNA was isolated from cartilage samples, following by being amplified, labeled and hybridized to Agilent human whole genome microarray chip.
This database contains microarray data of 40 different experimental conditions obtained through microarray analysis using the same custom microarray chip as described in this manuscript.
Expression profiling was performed using an Agilent human whole genome oligo microarray chip (4 × 44 K) (Agilent, USA).
Quantification was accomplished by flow cytometry and a flow-through microarray chip reader applying antibody microarrays for chemiluminescence sandwich immunoassays.
The replicate data were highly consistent when the same target was hybridized to two different sectors of the same microarray chip, to different microarray chips, or to the same microarray chip upon re-use (Fig. S2A).
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