The recently introduced new antigen microarray chip enables detection in parallel of the patterns of antibodies binding to hundreds of antigens, and so provides a system-level view of the antibody repertoire [1], [2], [3].
The potential of the cell microarray chip for the detection of malaria-infected erythrocytes was shown, it offering 10 100 times higher sensitivity than that of conventional light microscopy and easy operation in 15 min with purified erythrocytes.
Hybridization probes are widely used in many fields that require detection or monitoring of nucleic acids including gene microarrays, DNA chips, detection of gene translocation, monitoring intracellular mRNA and amplification progress in real-time PCR [ 4– 12].
To reduce the cost of detection, each microarray chip was designed and fabricated to accommodate 12 identical arrays which could be used for screening up to 12 different samples.
The advantage of deep sequencing over other detection methods, like northern blot analysis, RNAase protection assays, microarray chip techniques or real-time PCR, lies in the ability to detect low abundant and novel transcripts.
So, at least 2 4 days earlier detection of malaria is expected with our cell microarray chip than with conventional microscopic examination because of the 10 to 100 times higher sensitivity.
We have developed a spotted genome wide microarray chip with oligonucleotides printed in duplicate in order to minimise undesirable biases, thereby optimising detection of true differential expression.
The potential of a cell microarray chip system for the early diagnosis of malaria was shown, it allowing ultra-highly sensitive and accurate detection of malaria-infected erythrocytes in a short time.
Quantification was accomplished by flow cytometry and a flow-through microarray chip reader applying antibody microarrays for chemiluminescence sandwich immunoassays.
This database contains microarray data of 40 different experimental conditions obtained through microarray analysis using the same custom microarray chip as described in this manuscript.
