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This microarray chip covers 41,000 well-characterized human genes and transcripts.
This microarray chip covers 450,000 CpG sites per sample at single-nucleotide resolution, and each chip performed a total of 12 samples - one with 12 chronic periodontitis samples and the other with 11 control samples.
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This is the first comprehensive study on the effects of assisted epigenetic modifications on the transcriptome of bovine SCNT blastocysts studied with a microarray chip which covers the bovine preattachment transcriptome and hence allows the exploration of gene expression in bovine blastocysts obtained in different conditions.
The microarray chip used, EmbryoGENE®, covers most of the bovine pre-attachment transcriptome and hence allows the analysis of gene expression in bovine blastocysts.
Our data revealed several additional novel miRNAs, which have not been reported previously (Table 2), possibly because of a more comprehensive coverage of miRNAs in the miRNA microarray chips that cover 1205 human miRNAs and used in our study.
We used the Nimblegen "CpG island and promoter" microarray chip (Roche Nimblegen, WI) which covers all annotated Human Refseq gene promoters (24,659) and CpG islands (28,226) as annotated on the UCSC genome browser.
Genes covered by multiple probes on the microarray chip were collapsed to genes by selecting the probe yielding the highest signal (J-Express 2009 software; MolMine AS, Hafrsfjord, Norway).
The Agilent "105K" microarray chip was designed for maize genomic and transcript sequences in an effort to cover most maize genes with at least one 60-mer oligonucleotide probe.
Each microarray chip was hybridized to a single sample labeled with Cy3. Background subtraction and normalization were performed.
Quantification was accomplished by flow cytometry and a flow-through microarray chip reader applying antibody microarrays for chemiluminescence sandwich immunoassays.
To directly measure functional roles of uncultivated microbes, we developed Chip-stable isotope probing (SIP), a high-sensitivity, high-throughput SIP method performed on a phylogenetic microarray (chip).
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