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Digital PCR distributed the DNA samples onto a microarray chip containing thousands of reaction chambers, and quantified the DNA copy numbers by analysing the number of positive signals without any calibration curves needed.
Fluorescein-labeled miRNA [36] were used for hybridization on each miRNA microarray chip containing 509 probes in triplicate, corresponding to 435 human (including 122 predicted miRNAs), 261 mouse, and 196 rat miRNAs found in the miRNA Registry (http://microrna.sanger.ac.uk/sequences/; accessed Oct. 2005) or collected from a published paper [37].
A cDNA microarray chip containing 2,950 cDNA spots representing 960 SSH clones was constructed as previously described (29, 30).
A microarray chip containing probes to all the genes of isolates Hib 10810 and NTHi R2866 was designed.
Briefly, labeled targets from 5 μg of total RNA were used for hybridization on microarray chip containing 3766 probes in triplicate, including 627 human precursors miRNA genes.
In order to perform a more comprehensive search for genes regulated by telomere length, we constructed a microarray chip containing many newly identified telomere-proximal genes.
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The HG-U133A microarray chip contains 22,283 probe sets, from which a subset was selected for correlation analysis.
Each miRNA microarray chip contained 1,199 probes, including 703 identified human miRNAs and 393 predicted miRNAs.
This microarray chip contains 28 869 oligonucleotide probes for known and unknown genes.
Each microarray chip contained 277 control probes and 32 878 probes representing 29 098 genes.
Each microarray chip contained 39 200 polynucleotide data, of which 22 184 were mapped to the latest draft of the human genome.
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