Each microarray block contained a maximum of 80 punches and duplicates of each tumour were produced.
One mm diameter cores were generated using a semiautomatic arrayer (Alphelys Minicore2, Plaisir, France) and each tissue microarray block contained up to 165 cores with a total of 980 spots, with 5 spots per case.
Tissue microarray blocks containing breast cancer specimens obtained from 210 patients were constructed.
Tissue microarray blocks containing cores from 116 primary GC cases (TMA1) were constructed as described previously (Salto-Tellez et al, 2007).
The microarray blocks contained 97 cores with 59 samples of invasive breast cancer tissues with the rest of the cores representing normal breast tissue.
Each tissue microarray (TMA) block contained a single 0.6 mm core sampled from representative paraffin blocks from each patient.
Tissue microarray (TMA) blocks containing 2 cores of 0.6 mm from representative tumour tissue from all patients were sectioned at 4 μm and transferred to Superfrost + slides.
Sections from tissue microarray (TMA) blocks containing 849 CRC (stages I IV) and matching normal tissue samples were obtained from the West Australian Research Tissue Network, Department of Radiation Oncology, Sir Charles Gairdner Hospital.
Tissue microarray (TMA) blocks containing cores from 98 breast cancer patients were constructed, as described previously, under institutional ethics committee approval with consent for the tissue microarray program (NUS-IRB 05-017) [ 1, 16, 17] and used for the analysis.
From tissue microarray (TMA), blocks containing primary breast cancer tissues of (Aubele et al, 2007) 5 μm thick sections were cut, deparaffinised, and subjected to antigen retrieval by microwave cooking in 0.01 M citrate buffer (pH 6.0) at 1000 W for 30 min.
