Such RNA microarrays assess the quantity of all the RNA transcripts in the genome, which is specific to tissue, age, and state.
Gene expression profiling to elucidate response to stressors, based on both targeted gene 'biomarker' approach (e.g. qRT-PCR of biomarkers known to associate with disease, stress, environmental adaptation; Elder et al. 2008 ) and genome-based approaches [e.g., microarrays assessing the activity of 10s of thousands of genes or NGS of RNA transcripts (RNA-Seq) (Salem et al. 2012 )].
In contrast, microarray analysis assesses the transcript abundance of tens of thousands of genes, thus it has required the application of statistical methods to normalise the distribution of signals and also requires correction for large samples sets, so called false discovery rate correction [ 3, 4].
We also developed a tool for quality control of cDNA microarrays that assesses the technical quality of an ImmunoChip.
We used a custom microarray to assess the levels of UCE transcription during mouse development and integrated these data with published microarray and next-generation sequencing datasets as well as with newly produced PCR validation experiments.
Recently, Marques et al. [ 22, 71] used hybridization-based microarray to assess the global transcriptome of the whole CP following chronic peripheral LPS stimulation.
In this study we utilised a whole genome long oligonucleotide chicken microarray to assess the utility of cross species hybridisation (CSH).
Initially, we used microarray to assess the gene profiles of E2 compared with two endocrine disruptors: BPA, which is polymerized to produce polycarbonate plastics, and HPTE, which is a metabolite of the pesticide methoxychlor.
Application of oligonucleotide microarrays to assess the biological effects of neoadjuvant imatinib mesylate treatment for localized prostate cancer.
To further investigate the role of CtBP1 in colorectal cancer, we used tissue microarrays to assess the levels of the CtBP1 protein in normal mucosa, as well as in adenocarcinoma tissue obtained from 75 patients with colorectal cancer (Figure 4, E and F).
