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Recently, it has been shown by microarray assays that many of the Brachypodium CBF genes are cold-responsive [ 30].
We developed a system for assessing technical performance with microarray assays that involves two biologically complex samples (mixed tissue ratiometric controls (MTRC)) that are representative of experimental samples [ 14].
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A procedure to optimize a validation experiment, conditional upon an existing microarray assay that was not optimized for two-stage testing, is also introduced.
The platform includes a TaqMan real-time PCR designed for the universal detection of pestiviruses and a microarray assay that can use the amplicons produced in the real-time PCR to identify the specific pestivirus.
In this study, we demonstrate that serum miRNAs can be used to discriminate between cancer patient sera and normal donor sera, using a simple microarray assay that requires no amplification step.
For example, MammaPrint [ 1- 3], a DNA microarray assay that uses frozen tissue, is based on a 70-gene prognostic signature.
In our study, we detected six miRNA families (miR408/482/827/397/398/2118) in the microarray assay that were not present in the Solexa sequencing data (Additional file 8, Additional file 9).
Described here is a microarray assay that accurately identifies and quantifies T-cell repertoires for VB17 – JB2.7 rearrangements possessing IRSS as the core amino acid sequence.
In this report, we describe the development of a new NP-based genomic microarray assay that specifically identifies H5N1 viral nucleic acid and simultaneously provides subtype identification of influenza A virus in the absence of target amplification procedures such as RT-PCR.
We compared the ratio of Cy5/Cy3 intensity of each clone obtained from the regular cDNA microarray assays to that obtained from the SSH/microarray assays.
The results of Pol II chromatin immunoprecipitation whole genome microarray assays suggest that paused Pol II complexes are formed on approximately 10% of genes in the blastoderm stage Drosophila embryo [4].
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