The detection of eight RNA standards was investigated using common microarray and RT-qPCR approaches- Agilent one-colour 4 × 44 K microarrays and Taqman®-based real-time PCR using ABI 7900HT real-time PCR system, incorporating in- house designed oligonucleotide probes and assays respectively (for sequences, see Additional File 1).
A total of 69 and 701 genes differentially expressed in human hepatoma were finally identified (based on the data derived from the three tissue pairs in duplicate with log2 > 1 or < -1, and P < 0.01) by the regular cDNA microarray assays and by the SSH/microarray assays, respectively.
The microarray assays were carried out by Takara Bio. Inc. (Otsu, Japan) using products for microarray analysis manufactured by Agilent Technologies, according to the manufacturer's protocols.
Probing microarray assays in the presence of a hybridization mix retrieves precious information on hybridization kinetics.
Microarray assays could be informative in this regard.
VMM and CPP conducted cDNA microarray assays and preliminary analysis.
Afterwards, 300 ng aliquots of Cy3 and Cy5-labeled cRNAs from syngeneic and allogeneic skin explant assays, respectively, were mixed and hybridized to the microarrays.
In both studies, complementary DNA microarray assays of gene expression (10 163 probes for 9878 unique genes and 24 158 probes for 23 031 unique genes, respectively), along with (right-censored) survival data, were available for and patients, respectively.
We got microarray assays (GEO: GSE14888) of mouse 3T3-L1 adipocytissuesue cultures fed by metformin, then ran our method and the distance comparison method respectively, and presented the results in Table 3.
All samples were used for microarray assays after adjustment to a minimum concentration (50 ng/μl).
