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For example, currently available microarray assays require relatively large samples and complex sample preprocessing.
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The conventional microarray assay requires target amplification by PCR and incorporation of a fluorescent dye- or biotin-conjugated nucleotide into PCR products prior to hybridization.
While globin reduction assays have been shown to overcome these effects when used in conjunction with Affymetrix microarrays [ 7] and the standard Illumina direct hybridization assay [ 8, 9], globin reduction assays require large amounts of total RNA [ 7], fail to completely eliminate globin transcripts [ 7], and may induce distinct gene expression profiles [ 10].
However, all of these microarray based assays require two or more enzymatic amplification steps of influenza viral RNA prior to hybridization.
The microarray assays were carried out by Takara Bio. Inc. (Otsu, Japan) using products for microarray analysis manufactured by Agilent Technologies, according to the manufacturer's protocols.
Probing microarray assays in the presence of a hybridization mix retrieves precious information on hybridization kinetics.
Considering that numerous assays require a certain degree of restriction on probe design (e.g., regarding position in the genome), there could be much to gain by employing multiple stringencies, not only while developing the assay, but even during the actual use of the microarray.
Microarray assays could be informative in this regard.
VMM and CPP conducted cDNA microarray assays and preliminary analysis.
In this study, we demonstrate that serum miRNAs can be used to discriminate between cancer patient sera and normal donor sera, using a simple microarray assay that requires no amplification step.
Different from mRNA expression microarray, microRNA microarray requires another important characteristic in the assay.
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