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For example, both lncRNA and mRNA of ADAMTS15 were down-regulated in microarray assays; however, the lncRNA was down- but the mRNA was up-regulated in RT-qPCR.
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Probing microarray assays in the presence of a hybridization mix retrieves precious information on hybridization kinetics.
In all assays, we performed microRNA microarray assays according to the manufacturer's protocols available in April , 2008
Microarray assays could be informative in this regard.
VMM and CPP conducted cDNA microarray assays and preliminary analysis.
miR-494, miR-296, and miR-99a are the three miRNAs that exhibit the most discrepant correlation values and slopes between the qPCR and microarray assays (Fig. 2); however, if the measurement of miR-99a in placenta is omitted, the slope for this miRNA becomes -0.844 with R = -0.929 (see plot in Fig. 1).
However, they can be approximated using abundant expression datasets, for example utilizing normalized variation among microarray assays between replicates of populations [ 42, 43].
However, Glyma19g42240 and Glyma19g42460 (Histone-like transcription factors) had suppressed transcript levels at 48 hai in microarray assays [ 10], but not in the qRT-PCR assays.
Liquid microarray assays may therefore be added to the list of methods suitable for HCV genotyping.
EW performed the microarray assays.
CC and TW performed the microarray assays.
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