Although we identified 122 miRNAs (96 conserved + 26 non-conserved) and 64 miRNA*s, they showed a diverse range of abundance, and only a few miRNA families dominated in the miRNA library and microarray assay data.
Based on the knowledge gained from the microarray assay (data not shown) on cell fusion, custom made dot blot arrays were employed to verify whether the up-regulated genes on fused cell hybrids are biologically relevant in the development of doxorubicin resistance.
CH conducted experimental design, supervised microarray assays, conducted data interpretation, and contributed to the manuscript.
Hence, as for the microarray assays, we analyzed data from a total of 18 replicates at least (3 biological replicates × 3 within plate technical replicates × 2 technical replicates i.e. repeated measurements) obtained from each real time PCR experiment.
To understand how Tag-Seq compares with microarray assays, we compared expression data generated from the same human sample assayed by both Tag-Seq and by an Affymetrix Human Genome U133 Plus 2.0 microarray (supplementary fig. 1, Supplementary Material online).
The minor problem, from the outside, seems like the most significant, and that is the sheer quantity of data that microarray assays produce.
DP had overall responsibility for the execution of the microarray assays and statistical analyses of the data.
Taken together, these results lead us to suggest that the data from our microarray assays represented meaningful gene expression patterns that can be verified by independent gene expression assay systems.
KFL and YFW made the most important contributions to the manuscript, they performed most part of this study, including samples preparation, microarray assays and data analysis, and also writing the manuscript.
AaAQP6 is closely related to Drosophila CG12251 and vertebrate AQPs 11 and 12. First, we analyzed AQP expression data from microarray assays performed with probes from RNAs isolated from whole mosquito females (Figure 2).
